William Makis MD:
“In this study, we assessed the cell-killing potential of PDT using methylene blue (MB-PDT) in three breast epithelial cell lines… Cells were incubated in the absence or presence of MB and irradiated or not at 640 nm with 4.5 J/cm2”
“We observed that MB-PDT differentially induces massive cell death of tumour cells. Non-malignant cells were significantly more resistant to the therapy compared to malignant cells”
“The highest sensitivity to MB-PDT was observed in MDA-MB-231 cells, used as a model of TNBC (Triple Negative Breast Cancer), a tumour subtype for which there are no targeted treatments”
TNBC cells have lowest reduced glutathione levels compared to all other cell lines – this could be related to the inability of these cells to cope with MB-PDT-induced oxidative stress.
My Take…Highlights:
Methylene Blue targets breast cancer cells but not normal breast cells
Methylene Blue Photodynamic Therapy induces massive cell death of breast cancer cells but only weakly affects normal cells
Triple Negative Breast Cancer cells are most sensitive to killing by Methylene Blue
Multiple cell killing mechanisms are at play but don’t involve mitochondria…Methylene Blue localized not in mitochondria but in lysosomes.
Methylene Blue could be used as an adjunct to breast cancer surgery to “eliminate microscropic residual malignant cells in the post-surgical tumor bed and prevent local as well as metastatic recurrence without affecting normal tissues”
Methylene Blue Photodynamic Therapy could replace Radiation Treatment after breast cancer surgery. (!!)
This was a huge discovery. It was then promptly buried and never spoken of in Oncology again.
Because there is no money in Methylene Blue. 🤔
Those cancer cell killing graphs are stunning!
Have a look! 100% Cancer Cell killing in some cases…Especially for Triple Negative Breast Cancer!!
(blue bars are normal breast cells, black are breast cancer, red are TNBC)
[Via: makismd.substack.com]